Difference between revisions of "Part:BBa K3110043:Design"
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===Source=== | ===Source=== | ||
− | sfGFP was obtained from the Biobrick. | + | sfGFP was obtained from the Biobrick. J23117+B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extention) PCR. |
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===References=== | ===References=== |
Latest revision as of 18:50, 16 October 2019
J23117 Strong RBS sfGFP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 66
Design Notes
None.
Source
sfGFP was obtained from the Biobrick. J23117+B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extention) PCR.