Difference between revisions of "Part:BBa K3257119"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3257119 short</partinfo>
 
<partinfo>BBa_K3257119 short</partinfo>
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<span class='h3bb'>Sequence and Features</span>
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<partinfo>BBa_K3257119 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3257119 SequenceAndFeatures</partinfo>
  

Revision as of 16:43, 16 October 2019

lox5171-truncated mCherry-loxP

This part is a truncated mCherry fused two lox sites on both sides. It is used for the verification of the homologous recombination conducted by Cre. We purposely have not added a promoter or terminator in this composite part because, after homologous recombination, a wild-type mCherry will be recombined into this locus and we will be unable to detect red fluorescence since wild-type mCherry cannot be expressed at all.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 392
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 392
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 392
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 392
  • 1000
    COMPATIBLE WITH RFC[1000]