Difference between revisions of "Part:BBa K3257119"
(No difference)

Revision as of 16:42, 16 October 2019


lox5171-truncated mCherry-loxP

This part is a truncated mCherry fused two lox sites on both sides. It is used for the verification of the homologous recombination conducted by Cre. We purposely have not added a promoter or terminator in this composite part because, after homologous recombination, a wild-type mCherry will be recombined into this locus and we will be unable to detect red fluorescence since wild-type mCherry cannot be expressed at all.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 392
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 392
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 392
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 392
  • 1000
    COMPATIBLE WITH RFC[1000]