Difference between revisions of "Promoters/Catalog"

Revision as of 22:50, 7 October 2008

The registry has a large collection of Promoters available. We categorize promoters using two different parameters. First, most promoters are designed for a particular RNA polymerase holoenzyme, e.g E. coli RNA polymerase with σ70, or T7 RNA polymerase. Second, promoters may be either unregulated (constitutive), positively regulated (inducible) or negatively regulated (repressible). So for example, if you want express a gene in growing E. coli at a with little expression before hand, you might choose a lacI repressible σ70 promoter.

The value of the parts in this catalog increases the more you use them and report your results so please remember to contribute back any data (positive or negative!) that you obtain for any promoters in the catalog.

Regulation		RNA Polymerase
		colspan="2"\|E. coli	T7 RNA polymerase
		σ⁷⁰	σ^S
stuff	Inducible	See X promoters	See X promoters	See X promoters
	Constitutive	See X promoters	See X promoters	See X promoters
	Repressible	See X promoters	See X promoters	See X promoters
(+/-) image	Multiple	See X promoters	See X promoters	See X promoters

//Promoter/Coli/Sigma70/Constitutive
//Promoter/Coli/Sigma70/Repressible
//Promoter/Coli/Sigma70/Inducible
//Promoter/T7/Constitutive
//Promoter/T7/Repressible

Bacterial promoters
- E. coli promoters
  - σ70 promoters
    - Constitutive promoters
    - Repressible promoters
    - Activatable promoters
Eukaryotic promoters
- Yeast promoters
Phage promoters
- T7 promoters
  - Constitutive T7 promoters
  - Regulatable T7 promoters

@@ Line 3: / Line 3: @@
 The value of the parts in this catalog increases the more you use them and report your results so please remember to contribute back any data (positive or negative!) that you obtain for any promoters in the catalog.
-* rackham_constitutive_prokaryotic_RBS
-** //RBS/Custom/Constitutive/Rackham
+{|{{Table}} width="100%"
-* issacs_regulated_prokaryotic_RBS
+!colspan="2" rowspan="3"|Regulation
-** //RBS/Prokaryotic/Regulated/Issacs Family
+!colspan="3"|RNA Polymerase
-* community_constitutive_prokaryotic_RBS
+|-
-** //RBS/Prokaryotic/Constitutive/Community
+|align="center"|colspan="2"|''E. coli''
-* anderson_constitutive_prokaryotic_RBS
+|align="center"|T7 RNA polymerase
-** //RBS/Prokaryotic/Constitutive/Anderson
+|-
+|align="center"|&sigma;<sup>70</sup>
+|align="center"|&sigma;<sup>S</sup>
+|
+|-
+|width="50pt" rowspan="3"|stuff
+|width="50pt" |Inducible
+|align="center"| See X promoters
+|align="center"| See X promoters
+|align="center"| See X promoters
+|-
+|Constitutive
+|align="center"| See X promoters
+|align="center"| See X promoters
+|align="center"| See X promoters
+|-
+|Repressible
+|align="center"| See X promoters
+|align="center"| See X promoters
+|align="center"| See X promoters
+|-
+|(+/-) image
+|Multiple
+|align="center"| See X promoters
+|align="center"| See X promoters
+|align="center"| See X promoters
+|-
+|}
   //Promoter/Coli/Sigma70/Constitutive