Difference between revisions of "Part:BBa K143033"

m
m
 
(7 intermediate revisions by 4 users not shown)
Line 2: Line 2:
 
<partinfo>BBa_K143033 short</partinfo>
 
<partinfo>BBa_K143033 short</partinfo>
  
−
Transcriptional regulator proteins generally regulat trancription by binding to specific operator sequences around the transcription start site. These proteins can affect transcription positively (activators) or negatively (repressors). In addition, many of these regulatory proteins can be regulated themselves by the presence of chemcials and compounds, such as the inducers IPTG.
+
<br>
  
−
LacI is the regulator protein for the lactose operon in ''E.coli'' and the hyper-spank promoter of ''B. subtilis''<cite>#1</cite>(<bbpart>BBaK143015</bbpart>) and is responsible for ensuring there is no expression through these promoters in the absence of lactose (or IPTG). LacI is not endogenous to ''B. subtilis'' so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes.  
+
Transcriptional regulator proteins generally regulate trancription by binding to specific operator sequences around the transcription start site. These proteins can affect transcription positively (activators) or negatively (repressors). In addition, many of these regulatory proteins can be regulated themselves by the presence of chemicals and compounds, such as the inducers IPTG.  
  
−
This version of LacI lacks a Lva degradation tag and has a small(3 amino acid) N-terminal deletion relative to the current registry LacI (<bbpart>BBa_C0012</bbpart)> and its derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with ''B. subtilis'' though both forms are found in ''E.coli'' (in differing strains).
+
LacI is the regulator protein for the lactose operon in ''E. coli'' and the hyper-spank promoter of ''B. subtilis'' <cite>#1</cite> (<bbpart>BBa_K143015</bbpart>) and is responsible for ensuring there is no expression through these promoters in the absence of lactose (or IPTG). LacI is not endogenous to ''B. subtilis'' so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes.  
  
−
LacI can be used in conjunction with the '''lac operon promoter''' (<bbpart>BBa_K143015</bbpart>), where the LacI will act as a reciever for an IPTG input to result in an '''Polymerases per second''' (PoPS) output.
+
This version of LacI lacks an Lva degradation tag and has a small (3 amino acid) N-terminal deletion relative to the current registry LacI (<bbpart>BBa_C0012</bbpart>) and its derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with ''B. subtilis'' though both forms are found in ''E. coli'' (in differing strains).
  
 +
LacI can be used in conjunction with the '''lac operon promoter''' (<bbpart>BBa_K143015</bbpart>), where the LacI will act as a receiver for an IPTG input to result in a '''polymerases per second''' (PoPS) output.
 +
<br>
 +
<br>
 +
<br>
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===
Line 22: Line 26:
 
<partinfo>BBa_K143033 parameters</partinfo>
 
<partinfo>BBa_K143033 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
===References===
 +
 +
<biblio>
 +
#1 pmid=16166525
 +
</biblio>

Latest revision as of 14:16, 7 October 2008

LacI (Lva-, N-terminal deletion) regulatory protein


Transcriptional regulator proteins generally regulate trancription by binding to specific operator sequences around the transcription start site. These proteins can affect transcription positively (activators) or negatively (repressors). In addition, many of these regulatory proteins can be regulated themselves by the presence of chemicals and compounds, such as the inducers IPTG.

LacI is the regulator protein for the lactose operon in E. coli and the hyper-spank promoter of B. subtilis #1 (BBa_K143015) and is responsible for ensuring there is no expression through these promoters in the absence of lactose (or IPTG). LacI is not endogenous to B. subtilis so LacI will need to be expressed in the host in order for the hyper-spank promoter to be regulated. In the presence of IPTG or lactose, the LacI tetramer is unable to bind DNA and so transcription resumes.

This version of LacI lacks an Lva degradation tag and has a small (3 amino acid) N-terminal deletion relative to the current registry LacI (BBa_C0012) and its derivatives. The N-terminal deletion appears to be common to most of the LacI genes used in conjunction with B. subtilis though both forms are found in E. coli (in differing strains).

LacI can be used in conjunction with the lac operon promoter (BBa_K143015), where the LacI will act as a receiver for an IPTG input to result in a polymerases per second (PoPS) output.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

<biblio>

  1. 1 pmid=16166525

</biblio>