Difference between revisions of "Part:BBa K2936007"
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Revision as of 16:05, 16 October 2019
LacZ alpha
An alpha peptide chain encoded by a gene LacZ
Description
Short description: Reporter gene.
The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) ).
Source
E. coli
Design consideration
LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
Mechanism
