Difference between revisions of "Part:BBa K3264009"

Revision as of 16:01, 16 October 2019

NT3WCT

cloth, and aerospace fields. However, spider breeding is not applicable due to spider's fierce behavior. The current approach is to produce recombinant spidroins (silk proteins) from other chassis and spin them into silk. NT-W-CT is the common architecture in two main types of spider silk proteins: major ambulate spidorins(MaSps) and minor ampullate spidorins (MiSps). NT is a non-repetitive N-terminal domain, as well as CT, the C-terminal domain. In between them is an extensive repetitive region-Rep In the storage of spider silk protein, NT is a monomeric and highly soluble region in neutral pH, and it provides the solubility of the entire spidorin. NT can form stable dimers when pH decreases in the spinning duct. On the other side during the decrease of pH, the CT gets disrupted and unfold to turn in the beta-sheet amyloid-like fibrils. It is hypothesized that structural change of CT precipitate the change of the repetitive region to beta-sheet conformation. Enable to get a step closer to unobtainable size (556 kDa), containing 192 repeat motifs of the Nephila clavipes dragline spidroin, we have developed a synthetic biology approach where we combine standardized DNA part assembly and split the interinmediated ligation to produce recombinant spidroins of NT-3W-CT, in this case, extra a 2W are added to the NT-2Rep-CT and was expected to have a better mechanical performance after spinning.

Sequence and Features