Difference between revisions of "Part:BBa K3093000"
Still Water (Talk | contribs) |
Still Water (Talk | contribs) |
||
| Line 4: | Line 4: | ||
"Chitobiose regulator," ChbR, is a dual transcriptional factor that regulates an operon (chb) involved in transport and degradation of the -glucoside N,N'-diacetylchitobiose (chitobiose) . [More information is available at EcoCyc: EG10143]. | "Chitobiose regulator," ChbR, is a dual transcriptional factor that regulates an operon (chb) involved in transport and degradation of the -glucoside N,N'-diacetylchitobiose (chitobiose) . [More information is available at EcoCyc: EG10143]. | ||
| + | |||
The sequence of chbR was obtained from E.coli K12 MG1655 via PCR.Then we performed inverse PCR to achieve the site-directed mutation of amino acids of chbR. According to the literature, we chose three site-directed mutation : chbRN238S | The sequence of chbR was obtained from E.coli K12 MG1655 via PCR.Then we performed inverse PCR to achieve the site-directed mutation of amino acids of chbR. According to the literature, we chose three site-directed mutation : chbRN238S | ||
Revision as of 15:37, 16 October 2019
chbR N238S Y30C
"Chitobiose regulator," ChbR, is a dual transcriptional factor that regulates an operon (chb) involved in transport and degradation of the -glucoside N,N'-diacetylchitobiose (chitobiose) . [More information is available at EcoCyc: EG10143].
The sequence of chbR was obtained from E.coli K12 MG1655 via PCR.Then we performed inverse PCR to achieve the site-directed mutation of amino acids of chbR. According to the literature, we chose three site-directed mutation : chbRN238S
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]