Difference between revisions of "Part:BBa K2449003"
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<partinfo>BBa_K2449003 parameters</partinfo>
 
<partinfo>BBa_K2449003 parameters</partinfo>
 
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=ECUST_China 2019 characterization=
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In ECUST_China 2019 characterization, we used Cep 94A to degrade cellobiose to glucose and glucose-phosphate. To characterize the Cep 94A specifically, we performed SDS-PAGE and measured enzyme activity.
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SDS-PAGE revealed that Cep 94A was expressed successfully (about 91kDa). After that, we sonicated E.coli and extract Cep 94A, using DNS assay to measure the enzyme activity.

Revision as of 15:36, 16 October 2019


cep94A

This is the so called cellobiose phosphorylase (Cep94A), which phosphorylates the cellobiose β-linkage resulting in its breakdown to D-glucose and α-D-glucose-1-phosphate. It is used in the following biobrick BBa_K2449004 For more info visit https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294459/

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 846
    Illegal NgoMIV site found at 1942
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 108


ECUST_China 2019 characterization

In ECUST_China 2019 characterization, we used Cep 94A to degrade cellobiose to glucose and glucose-phosphate. To characterize the Cep 94A specifically, we performed SDS-PAGE and measured enzyme activity.

SDS-PAGE revealed that Cep 94A was expressed successfully (about 91kDa). After that, we sonicated E.coli and extract Cep 94A, using DNS assay to measure the enzyme activity.