Difference between revisions of "Part:BBa K3286011"
Line 9: Line 9:
 
<p>Anderson promoter J23109: https://parts.igem.org/Part:BBa_J23119</p>
 
<p>Anderson promoter J23109: https://parts.igem.org/Part:BBa_J23119</p>
 
<p>Tac promoter: https://parts.igem.org/Part:BBa_K2933026</p>
 
<p>Tac promoter: https://parts.igem.org/Part:BBa_K2933026</p>
−
<p>sgRNA expression cassette: https://parts.igem.org/Part:BBa_K3286001)</p>
+
<p>sgRNA expression cassette: https://parts.igem.org/Part:BBa_K3286001</p>
 
<p>rho-independent terminator: https://parts.igem.org/Part:BBa_K3286002</p>
 
<p>rho-independent terminator: https://parts.igem.org/Part:BBa_K3286002</p>
  

Revision as of 15:25, 16 October 2019


sgRNA expression cassette (double target)

Double expression cassette of the sgRNA for Cas9 of Streptococcus pyogenes with non-targeting (NT) spacer.

For both sgRNAs an rho-independent terminator is used. One sgRNA is under control of the strong constitutive Anderson promoter J23109, the other under the also constitutive Tac promoter.

Anderson promoter J23109: https://parts.igem.org/Part:BBa_J23119

Tac promoter: https://parts.igem.org/Part:BBa_K2933026

sgRNA expression cassette: https://parts.igem.org/Part:BBa_K3286001

rho-independent terminator: https://parts.igem.org/Part:BBa_K3286002


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 197
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 197
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 197
  • 1000
    COMPATIBLE WITH RFC[1000]