Difference between revisions of "Part:BBa K3286011"

 
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Double expression cassette of the sgRNA for Cas9 of <em>Streptococcus pyogenes</em> with non-targeting (NT) spacer (https://parts.igem.org/Part:BBa_K3286001).
 
Double expression cassette of the sgRNA for Cas9 of <em>Streptococcus pyogenes</em> with non-targeting (NT) spacer (https://parts.igem.org/Part:BBa_K3286001).
  
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For both sgRNAs an rho-independent terminator (https://parts.igem.org/Part:BBa_K3286002) is used. One sgRNA is under control of the strong constitutive Anderson promoter J23109 (https://parts.igem.org/Part:BBa_J23119), the other under the also constitutive Tac promoter (https://parts.igem.org/Part:BBa_K2933026).  
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For both sgRNAs an rho-independent terminator is used. One sgRNA is under control of the strong constitutive Anderson promoter J23109, the other under the also constitutive Tac promoter.
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<p>Anderson promoter J23109: https://parts.igem.org/Part:BBa_J23119</p>
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<p>Tac promoter: https://parts.igem.org/Part:BBa_K2933026</p>
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<p>sgRNA expression cassette: https://parts.igem.org/Part:BBa_K3286003</p>
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<p>rho-independent terminator: https://parts.igem.org/Part:BBa_K3286002</p>
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:24, 16 October 2019


sgRNA expression cassette (double target)

Double expression cassette of the sgRNA for Cas9 of Streptococcus pyogenes with non-targeting (NT) spacer (https://parts.igem.org/Part:BBa_K3286001).

For both sgRNAs an rho-independent terminator is used. One sgRNA is under control of the strong constitutive Anderson promoter J23109, the other under the also constitutive Tac promoter.

Anderson promoter J23109: https://parts.igem.org/Part:BBa_J23119

Tac promoter: https://parts.igem.org/Part:BBa_K2933026

sgRNA expression cassette: https://parts.igem.org/Part:BBa_K3286003

rho-independent terminator: https://parts.igem.org/Part:BBa_K3286002


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 197
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 197
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 197
  • 1000
    COMPATIBLE WITH RFC[1000]