Difference between revisions of "Part:BBa K3040100"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | This gene comes from Pseudomonas sp. 7323 and the effective temperature range matches the environmint condition. | ||
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Revision as of 15:23, 16 October 2019
Lipase-Pseudomonas sp 7323.
Lipase from Antarctic cold-tolerant Pseudomonas sp. 7323 can catalyze the hydrolysis of triacylglycerols to glycerol and monoacylglycerols. We utilize the characteristic of lipase-having different catalytic activity under different temperature, to sense the temperature change. Lipase A reaches its catalytic activity peak at about 30℃, and decreases as temperature rise or drop. Furthermore, to purify our lipase A in an easier way, we design a His-tag sequence behind the lipase A.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 147
Illegal NgoMIV site found at 259
Illegal NgoMIV site found at 1294 - 1000COMPATIBLE WITH RFC[1000]