Difference between revisions of "Part:BBa K3286008"
AlexNiederau (Talk | contribs) |
|||
| Line 5: | Line 5: | ||
The expression dCas9 is under control of the constitutive TET promoter. Expression regulation is achieved by the lac operator located between dCas9 and pTET. In an un-induced state, the lacI protein is bound to the lac operator inhibiting expression of the dCas9. Upon addition of the inducer IPTG (or Lactose) the repressor lacI binds to the inducer and dCas9 is being expressed. | The expression dCas9 is under control of the constitutive TET promoter. Expression regulation is achieved by the lac operator located between dCas9 and pTET. In an un-induced state, the lacI protein is bound to the lac operator inhibiting expression of the dCas9. Upon addition of the inducer IPTG (or Lactose) the repressor lacI binds to the inducer and dCas9 is being expressed. | ||
| − | bi-directional terminator: https://parts.igem.org/Part:BBa_K3286006 | + | <p>bi-directional terminator: https://parts.igem.org/Part:BBa_K3286006</p> |
| − | dCas9: https://parts.igem.org/Part: | + | <p>dCas9: https://parts.igem.org/Part:BBa_K3286013</p> |
| − | lac operator: https://parts.igem.org/Part:BBa_K3286004 | + | <p>lac operator: https://parts.igem.org/Part:BBa_K3286004</p> |
pTET: https://parts.igem.org/Part:BBa_K3286005 | pTET: https://parts.igem.org/Part:BBa_K3286005 | ||
lacI promoter: https://parts.igem.org/Part:BBa_K2572009 | lacI promoter: https://parts.igem.org/Part:BBa_K2572009 | ||
Revision as of 14:37, 16 October 2019
IPTG inducible dCas9 expression module
The expression dCas9 is under control of the constitutive TET promoter. Expression regulation is achieved by the lac operator located between dCas9 and pTET. In an un-induced state, the lacI protein is bound to the lac operator inhibiting expression of the dCas9. Upon addition of the inducer IPTG (or Lactose) the repressor lacI binds to the inducer and dCas9 is being expressed.
bi-directional terminator: https://parts.igem.org/Part:BBa_K3286006
dCas9: https://parts.igem.org/Part:BBa_K3286013
lac operator: https://parts.igem.org/Part:BBa_K3286004
pTET: https://parts.igem.org/Part:BBa_K3286005 lacI promoter: https://parts.igem.org/Part:BBa_K2572009 lacI: https://parts.igem.org/Part:BBa_K143033 Prarthana terminator: BBa_K3286007
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1200
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3479
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]