Difference between revisions of "Part:BBa K2936001"
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<partinfo>BBa_K2936001 short</partinfo> | <partinfo>BBa_K2936001 short</partinfo> | ||
| − | + | The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. In E. coli, the PBAD promoter is adjacent to the PC promoter, which transcribes the araC gene in the opposite direction. | |
| + | The LacZ alpha gene is one of the structural genes present on the Lac Operon in E. coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene encoding β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) will be expressed. | ||
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| + | <img src="https://static.igem.org/mediawiki/parts/9/93/T--ZJUT-China--igem-part12tonglu.png" alt=""width="600px"> | ||
| + | </html> | ||
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| + | ===<h1>Usage and Biology</h1>=== | ||
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Revision as of 14:22, 16 October 2019
pBAD-LacZɑ
The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. In E. coli, the PBAD promoter is adjacent to the PC promoter, which transcribes the araC gene in the opposite direction.
The LacZ alpha gene is one of the structural genes present on the Lac Operon in E. coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene encoding β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) will be expressed.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 120
Illegal BamHI site found at 182 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]