Difference between revisions of "Part:BBa K2936001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | When the BAD promoter was transcribed by RNA polymerase, the β-galactosidase encoded by LacZ alpha gene could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color. | |
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===Source=== | ===Source=== | ||
Latest revision as of 14:19, 16 October 2019
pBAD-LacZɑ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 120
Illegal BamHI site found at 182 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When the BAD promoter was transcribed by RNA polymerase, the β-galactosidase encoded by LacZ alpha gene could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
Source
The pGLO plasmid