Difference between revisions of "Part:BBa K3253003:Design"
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | This sequence contains multiple restriction site that there can be wrong restriction cutting. Therefore, we add extra restriction site in order to make it be successfully cut and transferred. | |
| Line 13: | Line 13: | ||
===Source=== | ===Source=== | ||
| − | This gene comes from the genomic sequence of red chicken-feet cotton, and it is synthesized by the IGEM official. | + | This gene comes from the genomic sequence of red chicken-feet cotton, and it is synthesized by the IGEM official. The gene is often overexpressed in the plant in the conditions in which the plant receives mechanical damage. |
===References=== | ===References=== | ||
Ma, Yin-Ping, et al. “Cloning and Functional Analysis of Chitinase Gene GbCHI from Sea-Island Cotton (Gossypium Barbadense).” Hereditas (Beijing), vol. 34, no. 2, 2012, pp. 240–247., doi:10.3724/sp.j.1005.2012.00240. | Ma, Yin-Ping, et al. “Cloning and Functional Analysis of Chitinase Gene GbCHI from Sea-Island Cotton (Gossypium Barbadense).” Hereditas (Beijing), vol. 34, no. 2, 2012, pp. 240–247., doi:10.3724/sp.j.1005.2012.00240. | ||
Revision as of 14:14, 16 October 2019
Ghchi 6
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence contains multiple restriction site that there can be wrong restriction cutting. Therefore, we add extra restriction site in order to make it be successfully cut and transferred.
Source
This gene comes from the genomic sequence of red chicken-feet cotton, and it is synthesized by the IGEM official. The gene is often overexpressed in the plant in the conditions in which the plant receives mechanical damage.
References
Ma, Yin-Ping, et al. “Cloning and Functional Analysis of Chitinase Gene GbCHI from Sea-Island Cotton (Gossypium Barbadense).” Hereditas (Beijing), vol. 34, no. 2, 2012, pp. 240–247., doi:10.3724/sp.j.1005.2012.00240.