Difference between revisions of "Part:BBa K2936007"

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===<h1>Usage and Biology</h1>===
 
===<h1>Usage and Biology</h1>===
 
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  The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) )
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  Mechanism：
 
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===<h1>Characterize</h1>===
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  <img src="https://static.igem.org/mediawiki/parts/8/89/T--ZJUT-China--igem-part07jili.png" alt=""width="600px">
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LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
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  Form 1 The ABS350 of reaction solution of bacterial culture and ONPG
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<br>Mechanism:
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[[File:T--ZJUT-China--igem-part07jili.png]]
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===<h1>Experimental Results</h1>===
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<p>Form 1 The absorbance of different reaction solution at the wavelength of350 nm</p >
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   < img src="https://2019.igem.org/wiki/images/5/5a/T--ZJUT-China--igem-part07form.png" alt=""width="600px">
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   <img src="https://static.igem.org/mediawiki/parts/5/5a/T--ZJUT-China--igem-part07form.png" alt=""width="600px">
 
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   <img src="https://2019.igem.org/wiki/images/thumb/5/57/T--ZJUT-China--igem-part07reaction.jpg/800px-T--ZJUT-China--igem part07reaction.jpg" alt="" width="600px">
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   <img src="https://static.igem.org/mediawiki/parts/5/57/T--ZJUT-China--igem-part07reaction.jpg" alt=""width="600px">
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  <p>Figure 1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.</p >
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<b>Fig1.</b> The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to righ
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From the figure 7 the color of the third group is enough obvious compared to the background， and at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD650=0.5) to TMB was designed to be 1:10.
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Revision as of 13:48, 16 October 2019


LacZ alpha

An alpha peptide chain encoded by a gene LacZ


Usage and Biology

Mechanism：


Form 1 The ABS350 of reaction solution of bacterial culture and ONPG


Fig1. The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to righ

From the figure 7 the color of the third group is enough obvious compared to the background， and at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD650=0.5) to TMB was designed to be 1:10.