Difference between revisions of "Part:BBa K3002018"

Revision as of 13:29, 16 October 2019

Sp20 8xHis tag for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the sequence of the sp20 8xHis-tag (B5) and was built as a part of the Kaiser Collection. Combined with a secretion signal of the Kaiser Collection like BBa_K3002007 (cCA (B2)), BBa_K3002008 (GLE (B2)) or BBa_3002009 (ARS (B2)), an appropriate promoter (BBa_K3002001 (PSAD promoter (A1-B1)) or BBa_K3002031 (PAR promoter (A1-B1))) and a terminator (e.g. BBa_K3002006(RPL23 terminator)) your target protein is secreted efficiently and can be purified via a nickel-NTA column.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 <html>
-This basic part contains the sequence of the sp20 8xHis-tag (B5) and was built as a part of the Kaiser Collection. Combined with a secretion signal of the Kaiser Collection like <a href="https://parts.igem.org/Part:BBa_K3002007">BBa_K3002007 </a> (cCA (B2)), <a href="https://parts.igem.org/Part:BBa_K3002008">BBa_BBa_K3002008 </a> (GLE (B2)) or <a href="https://parts.igem.org/Part:BBa_3002009">BBa_3002009 </a> (ARS (B2)), an appropriate promoter (<a href="https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001</a> (PSAD promoter (A1-B1)) or <a href="https://parts.igem.org/Part:BBa_K3002031">BBa_K3002031</a> (PAR promoter (A1-B1))) and a terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_BBa_K3002006</a>(RPL23 terminator)) your target protein is secreted efficiently and can be purified via a nickel-NTA column.
+This basic part contains the sequence of the sp20 8xHis-tag (B5) and was built as a part of the Kaiser Collection. Combined with a secretion signal of the Kaiser Collection like <a href="https://parts.igem.org/Part:BBa_K3002007">BBa_K3002007 </a> (cCA (B2)), <a href="https://parts.igem.org/Part:BBa_K3002008">BBa_K3002008 </a> (GLE (B2)) or <a href="https://parts.igem.org/Part:BBa_3002009">BBa_3002009 </a> (ARS (B2)), an appropriate promoter (<a href="https://parts.igem.org/Part:BBa_K3002001">BBa_K3002001</a> (PSAD promoter (A1-B1)) or <a href="https://parts.igem.org/Part:BBa_K3002031">BBa_K3002031</a> (PAR promoter (A1-B1))) and a terminator (e.g. <a href="https://parts.igem.org/Part:BBa_K3002006">BBa_K3002006</a>(RPL23 terminator)) your target protein is secreted efficiently and can be purified via a nickel-NTA column.
 </html>