Difference between revisions of "Part:BBa K2984036"

Revision as of 13:16, 16 October 2019

L1c-PsaD-PETase-YFP-RBCS2

This part is composed of the PsaD promoter (BBa_K2984008), a B2-B2 linker (BBa_K2984034), the PETase enzyme (), a YFP mVenus marker (BBa_K2984024), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression of the PETase.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1915
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1244
Illegal PstI site found at 1915
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
Illegal XhoI site found at 968
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1915
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1915
Illegal NgoMIV site found at 2392
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2984036 short</partinfo>
-This part is composed of the PsaD promoter (BBa_K2984008), a B2-B2 linker (BBa_K2984034), the PETase enzyme (), a YFP mVenus marker (BBa_K2984024), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.
+This part is composed of the PsaD promoter (BBa_K2984008), a B2-B2 linker (BBa_K2984034), the PETase enzyme (), a YFP mVenus marker (BBa_K2984024), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express the PETase enzyme in C. reinhardtii. The YFP-mVenus tag can be used to detect the expression of the PETase.
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