Difference between revisions of "Part:BBa K3241029"

 
 
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<partinfo>BBa_K3241029 short</partinfo>
 
<partinfo>BBa_K3241029 short</partinfo>
  
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In R. eutropha cells, PHB is made through 3 steps.Two acetyl-CoA molecules made from carbohydrate converted to acetoacetyl-CoA by &#946;-ketothiolase by PhbA gene. Then acetoacetyl-CoA reductase encoded by PhbB gene catalyzes the reduction of acetoacetyl-CoA to (R)-3-hydroxybutyryl-CoA (3HB-CoA). Finally, PHB synthase encoded by PhbC gene catalyzes polymerization reaction of monomer molecules to PHB.
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This part is a new part, which consists of BBa_K3241000 BBa_K3241022 BBa_K3241112 BBa_K2800032 BBa_K2800013 BBa_K3241025
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. It is used to construct the PHB biosynthetic pathway in Zymomonas mobilis .
  
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[[File:T--HUBU_WUHAN--PHB_pathway_genes.png|400px|left|Fig.1 PHB pathway genes.]]
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 13:12, 16 October 2019


Ptet+PhbC+PhbA+PhbB

This part is a new part, which consists of BBa_K3241000 BBa_K3241022 BBa_K3241112 BBa_K2800032 BBa_K2800013 BBa_K3241025 . It is used to construct the PHB biosynthetic pathway in Zymomonas mobilis .

Fig.1 PHB pathway genes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 207
    Illegal NheI site found at 1083
    Illegal NheI site found at 2999
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 237
    Illegal NgoMIV site found at 2098
    Illegal NgoMIV site found at 2183
    Illegal NgoMIV site found at 2588
    Illegal NgoMIV site found at 2717
    Illegal AgeI site found at 342
    Illegal AgeI site found at 3121
    Illegal AgeI site found at 3793
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 163
    Illegal BsaI site found at 884
    Illegal BsaI site found at 2014