Difference between revisions of "Part:BBa K2982007:Design"

 
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===Design Notes===
 
===Design Notes===
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It have a XbaI restriction site that allow the cutting of part. It also contain linkage sequence fot PET-28a vector between the RE site and RBS, also between RBS and the GFP gene.
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It has a XbaI restriction site that allows the cutting of the part. It also contains linkage sequences of the PET-28a vector between the RE site and RBS, also between RBS and the GFP gene.
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===Source===
 
===Source===

Latest revision as of 12:46, 16 October 2019


Forward primer with BBa_J61124 for amplifying BBa_E0040


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It has a XbaI restriction site that allows the cutting of the part. It also contains linkage sequences of the PET-28a vector between the RE site and RBS, also between RBS and the GFP gene.

Source

iGEM registry

References