Difference between revisions of "Part:BBa K3039017:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The amino acid sequence was submitted to. | + | The amino acid sequence was submitted to Twist Bioscience who codon optimised the sequence for <I>E. coli</I>, ensuring that there were no forbidden restriction sites, BsaI or SapI, to allow for potential TypeIIS assembly. |
===Source=== | ===Source=== | ||
| − | + | Wild type amino acid sequence originates from <I>Ideonella sakaiensis</I>. Mutations have been directed by reference to the literature. Resulting DNA sequences were codon optimized by TWIST biosciences. | |
| − | Wild type amino acid sequence originates from Ideonella sakaiensis. Mutations have been directed by reference to the literature. Resulting DNA sequences were codon optimized by. | + | |
===References=== | ===References=== | ||
Latest revision as of 12:10, 16 October 2019
PETase Reconstructed Ancestor 1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The amino acid sequence was submitted to Twist Bioscience who codon optimised the sequence for E. coli, ensuring that there were no forbidden restriction sites, BsaI or SapI, to allow for potential TypeIIS assembly.
Source
Wild type amino acid sequence originates from Ideonella sakaiensis. Mutations have been directed by reference to the literature. Resulting DNA sequences were codon optimized by TWIST biosciences.