Difference between revisions of "Part:BBa K3096052"

 
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This CRISPR array codes for the gRNAs that target the plasmid which encodes the glucose dependent Exendin-4 expression system and is part of the CRISPR/Cas3 kill switch system to prevent the release of the probiotic. The gRNA will guide the Cas3 Nuclease to the plasmid DNA which will then be degraded.
 
This CRISPR array codes for the gRNAs that target the plasmid which encodes the glucose dependent Exendin-4 expression system and is part of the CRISPR/Cas3 kill switch system to prevent the release of the probiotic. The gRNA will guide the Cas3 Nuclease to the plasmid DNA which will then be degraded.
  
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===Sequence and Features===
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3096052 SequenceAndFeatures</partinfo>
 
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==References==
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#'''Xia, Peng-Fei, et al'''. "Synthetic genetic circuits for programmable biological functionalities." ''Biotechnology advances'' ('''2019''').
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#'''Terns, Michael P., and Rebecca M. Terns'''. "CRISPR-based adaptive immune systems." ''Current opinion in microbiology'' 14.3 ('''2011'''): 321-327.
 
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3096052 parameters</partinfo>
 
<partinfo>BBa_K3096052 parameters</partinfo>
 
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Latest revision as of 11:40, 16 October 2019

CRISPR Array targeting the Plasmid DNA of the glucose dependent Exendin-4 expression system

This CRISPR array codes for the gRNAs that target the plasmid which encodes the glucose dependent Exendin-4 expression system and is part of the CRISPR/Cas3 kill switch system to prevent the release of the probiotic. The gRNA will guide the Cas3 Nuclease to the plasmid DNA which will then be degraded.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

  1. Xia, Peng-Fei, et al. "Synthetic genetic circuits for programmable biological functionalities." Biotechnology advances (2019).
  2. Terns, Michael P., and Rebecca M. Terns. "CRISPR-based adaptive immune systems." Current opinion in microbiology 14.3 (2011): 321-327.