Difference between revisions of "Part:BBa K2924015"
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The promoter aldA was published as an oleic acid (C18:1) sensitive promoter enabling for the measurement of free fatty acids present in microorganisms. | The promoter aldA was published as an oleic acid (C18:1) sensitive promoter enabling for the measurement of free fatty acids present in microorganisms. | ||
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In <i>Escherichia coli</i> the growth on fatty acids requires many different proteins which are repressed by the transcriptional factor FadR.<sup>1</sup> The long chain acyl-CoA ester is an effector on the transcriptional factor FadR for the regulation of fatty acid metabolism.<sup>2</sup> | In <i>Escherichia coli</i> the growth on fatty acids requires many different proteins which are repressed by the transcriptional factor FadR.<sup>1</sup> The long chain acyl-CoA ester is an effector on the transcriptional factor FadR for the regulation of fatty acid metabolism.<sup>2</sup> | ||
| + | <i>Fig.1: Regulation of the fatty acid metabolism by the transcriptional factor FadR. FadR recognizes its cognate binding site (white), thereby repressing transcription. Upon binding of an Acyl-CoA to FadR, the promoter region is freed, enabling gene expression.</i> | ||
| − | After adding oleic acid (C18:1) many proteins showed an altered expression level and new proteins like AldA were synthesised. The promoter for aldA was also used for the production of green fluorescent protein (GFP).1 P<sub>aldA</sub> was isolated from the <i>Escherichia coli</i> wild type genome. | + | After adding oleic acid (C18:1) many proteins showed an altered expression level and new proteins like AldA were synthesised. The promoter for aldA was also used for the production of green fluorescent protein (GFP).1 P<sub>aldA</sub> was isolated from the <i>Escherichia coli</i> wild type genome.<!-- Add more about the biology of this part here |
| + | ===Usage and Biology=== | ||
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Revision as of 11:20, 16 October 2019
Promoter aldA from the Escherichia coli genome
The promoter aldA was published as an oleic acid (C18:1) sensitive promoter enabling for the measurement of free fatty acids present in microorganisms.
In Escherichia coli the growth on fatty acids requires many different proteins which are repressed by the transcriptional factor FadR.1 The long chain acyl-CoA ester is an effector on the transcriptional factor FadR for the regulation of fatty acid metabolism.2
Fig.1: Regulation of the fatty acid metabolism by the transcriptional factor FadR. FadR recognizes its cognate binding site (white), thereby repressing transcription. Upon binding of an Acyl-CoA to FadR, the promoter region is freed, enabling gene expression.
After adding oleic acid (C18:1) many proteins showed an altered expression level and new proteins like AldA were synthesised. The promoter for aldA was also used for the production of green fluorescent protein (GFP).1 PaldA was isolated from the Escherichia coli wild type genome.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]