Difference between revisions of "Part:BBa K2924015"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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In <i>Escherichia coli</i> the growth on fatty acids requires many different proteins which are repressed by the transcriptional factor FadR.<sup>1</sup> The long chain acyl-CoA ester is an effector on the transcriptional factor FadR for the regulation of fatty acid metabolism.<sup>2</sup> | In <i>Escherichia coli</i> the growth on fatty acids requires many different proteins which are repressed by the transcriptional factor FadR.<sup>1</sup> The long chain acyl-CoA ester is an effector on the transcriptional factor FadR for the regulation of fatty acid metabolism.<sup>2</sup> | ||
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After adding oleic acid (C18:1) many proteins showed an altered expression level and new proteins like AldA were synthesised. The promoter for aldA was also used for the production of green fluorescent protein (GFP).1 P<sub>aldA</sub> was isolated from the <i>Escherichia coli</i> wild type genome. | After adding oleic acid (C18:1) many proteins showed an altered expression level and new proteins like AldA were synthesised. The promoter for aldA was also used for the production of green fluorescent protein (GFP).1 P<sub>aldA</sub> was isolated from the <i>Escherichia coli</i> wild type genome. | ||
Revision as of 11:18, 16 October 2019
Promoter aldA from the Escherichia coli genome
The promoter aldA was published as an oleic acid (C18:1) sensitive promoter enabling for the measurement of free fatty acids present in microorganisms.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]