Difference between revisions of "Part:BBa K3102012"
 
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TEV protease, the common name for the 27 kDa catalytic domain of the Nuclear Inclusion is a (NIa) protein encoded by the tobacco etch virus (TEV). Its sequence specificity is far more stringent than that of factor Xa, thrombin, or enterokinase, TEV protease is a very useful reagent for cleaving fusion proteins.  
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__NOTOC__
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<partinfo>BBa_K3102012 short</partinfo>
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TEV protease is the 27 kDa catalytic domain of the Nuclear Inclusion is a (NIa) protein encoded by the tobacco etch virus (TEV). Its sequence specificity is far more stringent than the sequence of factor Xa, thrombin, or enterokinase. TEV protease is a very useful reagent for cleaving fusion proteins.  
  
 
Consequently, it’s an highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)(BBa_K3102041) and cleaves between the Gln and Gly/Ser residues.
 
Consequently, it’s an highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)(BBa_K3102041) and cleaves between the Gln and Gly/Ser residues.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3102012 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3102012 parameters</partinfo>
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Latest revision as of 10:53, 16 October 2019

TEV Protease

TEV protease is the 27 kDa catalytic domain of the Nuclear Inclusion is a (NIa) protein encoded by the tobacco etch virus (TEV). Its sequence specificity is far more stringent than the sequence of factor Xa, thrombin, or enterokinase. TEV protease is a very useful reagent for cleaving fusion proteins.

Consequently, it’s an highly specific cysteine protease that recognizes the amino-acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser)(BBa_K3102041) and cleaves between the Gln and Gly/Ser residues.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 492
    Illegal NgoMIV site found at 798
    Illegal AgeI site found at 153
  • 1000
    COMPATIBLE WITH RFC[1000]