Difference between revisions of "Part:BBa K3102028:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We mutated a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. We also mutated an Adenine into Guanine and a Cytosine in Thymine at the 2425bp and 2428bp position (A2425G & C2428T), respectively, because of the EcoRI enzyme. | + | We mutated the ACS operon sequence with a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. We also mutated an Adenine into Guanine and a Cytosine in Thymine at the 2425bp and 2428bp position (A2425G & C2428T), respectively, because of the EcoRI enzyme. (BBa_K3102013) |
===Source=== | ===Source=== | ||
| − | + | This sequence comes from E.coli and was found through Biocyc: | |
| + | https://biocyc.org/ECOLI/sequence-delimited?chromosome=COLI-K12&object=EG11448&up=0&down=2160&right=4287371&left=4283253&type=true | ||
| + | |||
| + | Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry. | ||
===References=== | ===References=== | ||
Gimenez R et al., The Gene yjcG, Cotranscribed with the Gene acs, Encodes an Acetate Permease in Escherichia coli, Journal of Bacteriology, 2003 Nov; 185(21):6448-55. | Gimenez R et al., The Gene yjcG, Cotranscribed with the Gene acs, Encodes an Acetate Permease in Escherichia coli, Journal of Bacteriology, 2003 Nov; 185(21):6448-55. | ||
Latest revision as of 10:36, 16 October 2019
T7-RBS-ACS operon-Ter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 408
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 837
Illegal AgeI site found at 2428 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 3702
Design Notes
We mutated the ACS operon sequence with a Cytosine into Thymine at the 1563bp position (C1563T) because of the Esp3I enzyme that we used for our Golden Gate Assembly (GGA) construct. We also mutated an Adenine into Guanine and a Cytosine in Thymine at the 2425bp and 2428bp position (A2425G & C2428T), respectively, because of the EcoRI enzyme. (BBa_K3102013)
Source
This sequence comes from E.coli and was found through Biocyc: https://biocyc.org/ECOLI/sequence-delimited?chromosome=COLI-K12&object=EG11448&up=0&down=2160&right=4287371&left=4283253&type=true
Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
References
Gimenez R et al., The Gene yjcG, Cotranscribed with the Gene acs, Encodes an Acetate Permease in Escherichia coli, Journal of Bacteriology, 2003 Nov; 185(21):6448-55.