Difference between revisions of "Part:BBa K3102040:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | We mutated an Adenine into Guanine at the | + | We mutated the MDH sequence with an Adenine into Guanine at the 786bp position (A786G) because of the EcoRI enzyme. (BBa_K3102018) |
===Source=== | ===Source=== | ||
Latest revision as of 10:13, 16 October 2019
T7-RBS-MDH-Ter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 299
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 143
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We mutated the MDH sequence with an Adenine into Guanine at the 786bp position (A786G) because of the EcoRI enzyme. (BBa_K3102018)
Source
This sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-721
Promoter (BBa_I719005) , RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
References
Sutherland P. et al., "Isolation and expression of the Escherichia coli gene encoding malate dehydrogenase.", J. Bacteriology, 163:1074-1079(1985)v