Difference between revisions of "Part:BBa K2965040"
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<partinfo>BBa_K2965040 short</partinfo> | <partinfo>BBa_K2965040 short</partinfo> | ||
| − | This part is a crRNA of FnCas12a, it is complementary to HPV16 L1 sequence. There is a 5' TTN PAM on the DNA strand opposite the target sequence. When | + | This part is a crRNA of FnCas12a, it is complementary to HPV16 L1 sequence. There is a 5' TTN PAM on the DNA strand opposite the target sequence. When FnCas12a is guided by this crRNA, once it recognizes its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity. |
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Latest revision as of 10:05, 16 October 2019
Fn-crRNA-HPV16-3
This part is a crRNA of FnCas12a, it is complementary to HPV16 L1 sequence. There is a 5' TTN PAM on the DNA strand opposite the target sequence. When FnCas12a is guided by this crRNA, once it recognizes its target, it will be activated and exhibits a “collateral effects” of promiscuous DNase activity.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]