Difference between revisions of "Part:BBa K3089021"
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This composite part is meant to express csgA-linker-mfp5 fusion genes under T7 promoter and purify CsgA-linker-Mfp5 recombinant proteins by His-tag affinity purification. CsgA is an amyloid-like protein encoded on the genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5(BBa_K1583002)is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion. This recombinant protein would self-assemble into fibrous bundles or films with adhesivepropertiesby displaying the mussel adhesion domains on the surface of amyloid scaffolds, which would be a promising new generation of bio-inspired adhesives for a wide range of applications. | This composite part is meant to express csgA-linker-mfp5 fusion genes under T7 promoter and purify CsgA-linker-Mfp5 recombinant proteins by His-tag affinity purification. CsgA is an amyloid-like protein encoded on the genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5(BBa_K1583002)is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion. This recombinant protein would self-assemble into fibrous bundles or films with adhesivepropertiesby displaying the mussel adhesion domains on the surface of amyloid scaffolds, which would be a promising new generation of bio-inspired adhesives for a wide range of applications. | ||
Revision as of 09:41, 16 October 2019
T7 promoter+csgA-linker-mfp5-His fusion protein
This composite part is meant to express csgA-linker-mfp5 fusion genes under T7 promoter and purify CsgA-linker-Mfp5 recombinant proteins by His-tag affinity purification. CsgA is an amyloid-like protein encoded on the genome of E.coli MG1655 providing mechanical cohesive strength and Mfp5(BBa_K1583002)is a mussel foot protein from Mytilus galloprovincialis responsible for interface adhesion. This recombinant protein would self-assemble into fibrous bundles or films with adhesivepropertiesby displaying the mussel adhesion domains on the surface of amyloid scaffolds, which would be a promising new generation of bio-inspired adhesives for a wide range of applications.
This part was designed based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres" (Zhong et al, 2014.)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 76
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]