Difference between revisions of "Part:BBa K3038007:Design"
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RFC10 design | RFC10 design | ||
Optimized for GC content | Optimized for GC content | ||
| − | 6His tag | + | 6His tag added at the Cterm part (bp 3159 to 3176) |
| − | + | ||
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 09:39, 16 October 2019
Cytochrome 102A1 BM3 Mutant S72Y V78A F87A _Cterm 6His
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 81
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1225
Illegal SapI.rc site found at 2248
Illegal SapI.rc site found at 2848
Design Notes
RFC10 design Optimized for GC content 6His tag added at the Cterm part (bp 3159 to 3176)
Source
sequence synthesis by IDT