Difference between revisions of "Part:BBa K3176000:Design"
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+ | Malone, C. L., Boles, B. R., Lauderdale, K. J., Thoendel, M., Kavanaugh, J. S., & Horswill, A. R. (2009). Fluorescent reporters for Staphylococcus aureus. Journal of microbiological methods, 77(3), 251–260. doi:10.1016/j.mimet.2009.02.011 |
Latest revision as of 09:39, 16 October 2019
mRFP for S.aureus
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 597
Illegal AgeI site found at 709 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
During the design of BBa_K3176000, we had to change the promoter of BBa_J04450 from LacI to pMK4Pprot.
Source
This part was adapted from the biobrick BBa_J04450
References
Malone, C. L., Boles, B. R., Lauderdale, K. J., Thoendel, M., Kavanaugh, J. S., & Horswill, A. R. (2009). Fluorescent reporters for Staphylococcus aureus. Journal of microbiological methods, 77(3), 251–260. doi:10.1016/j.mimet.2009.02.011