Difference between revisions of "Part:BBa K3102047:Design"
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RibA : E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11331 | RibA : E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11331 | ||
| − | |||
RibB: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG10465 | RibB: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG10465 | ||
| − | |||
RibD: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11321 | RibD: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11321 | ||
| − | |||
RibE: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11322 | RibE: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11322 | ||
| − | |||
RibC: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11406 | RibC: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11406 | ||
| − | |||
RibF: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11079 | RibF: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11079 | ||
| + | |||
Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry. | Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry. | ||
===References=== | ===References=== | ||
| + | Bacher A. et al., "Riboflavin synthases of Bacillus subtilis. Purification and properties." Biol. Chem. 255:632-637(1980) | ||
| + | |||
| + | Eberhardt S.M.R. et al., J"Cloning, sequencing, mapping and hyperexpression of the ribC gene coding for riboflavin synthase of Escherichia coli." . Biochem. 242:712-719(1996) | ||
| + | |||
| + | Lin Z, et al., "Metabolic engineering of Escherichia coli for the production of riboflavin". Microb Cell Fact. 2014;13(1):1–12. | ||
| + | |||
| + | Zhenquan Lin et al., “Metabolic engineering of Escherichia coli for the production of riboflavin," Microbial Cell Factories, 2014, 13:104. | ||
Latest revision as of 08:39, 16 October 2019
Electron transfer improvement (Riboflavin, RibF)
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 354
Illegal PstI site found at 3318 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 354
Illegal PstI site found at 3318 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1013
Illegal BglII site found at 2110 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 354
Illegal PstI site found at 3318 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 354
Illegal PstI site found at 3318
Illegal AgeI site found at 201
Illegal AgeI site found at 903
Illegal AgeI site found at 945
Illegal AgeI site found at 1488
Illegal AgeI site found at 1863
Illegal AgeI site found at 3819 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
none
Source
RibA : E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11331
RibB: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG10465
RibD: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11321
RibE: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11322
RibC: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11406
RibF: E.coli, sequence found through Biocyc https://biocyc.org/gene?orgid=ECOLI&id=EG11079
Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.
References
Bacher A. et al., "Riboflavin synthases of Bacillus subtilis. Purification and properties." Biol. Chem. 255:632-637(1980)
Eberhardt S.M.R. et al., J"Cloning, sequencing, mapping and hyperexpression of the ribC gene coding for riboflavin synthase of Escherichia coli." . Biochem. 242:712-719(1996)
Lin Z, et al., "Metabolic engineering of Escherichia coli for the production of riboflavin". Microb Cell Fact. 2014;13(1):1–12.
Zhenquan Lin et al., “Metabolic engineering of Escherichia coli for the production of riboflavin," Microbial Cell Factories, 2014, 13:104.