Difference between revisions of "Part:BBa K2927065"
 
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<partinfo>BBa_K2927065 short</partinfo>
 
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The sequence we designed is used for generating crRNA 5 and mutated crRNA 4. CrRNA 5 is used for African swine fever virus (ASFV) detection. Furthermore, mutated crRNA 4 is the sequence derived from crRNA 4 for negative control to test whether our purified Cas12a protein works functionally and normally. Kpn1 is the cutting site that helps us to separate crRNA 5 and mutated crRNA 4.
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This set of DNA fragments is used to express normal and mutated crRNA through T7 promoter. The normal crRNA cooperates with Cas12a to detect specific DNA sequence in African swine fever virus (ASFV), while mutation abolish the interaction between crRNA and target sequence, which is served as negative control to confirm the specificity of crRNAs.  
  
 
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Latest revision as of 07:11, 16 October 2019


T7 promoter-guide RNA-crRNA 5-mutated crRNA 4

This set of DNA fragments is used to express normal and mutated crRNA through T7 promoter. The normal crRNA cooperates with Cas12a to detect specific DNA sequence in African swine fever virus (ASFV), while mutation abolish the interaction between crRNA and target sequence, which is served as negative control to confirm the specificity of crRNAs.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]