Difference between revisions of "Part:BBa K143062:Design"
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<partinfo>BBa_K143062 short</partinfo> | <partinfo>BBa_K143062 short</partinfo> | ||
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<partinfo>BBa_K143062 SequenceAndFeatures</partinfo> | <partinfo>BBa_K143062 SequenceAndFeatures</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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LacI was identified from the pDR111 ''B. subtilis'' integration vector. The double terminator is the most commonly used registry terminator. | LacI was identified from the pDR111 ''B. subtilis'' integration vector. The double terminator is the most commonly used registry terminator. | ||
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===Source=== | ===Source=== | ||
LacI was produced by PCR cloning using Pfu form the ''B. subtilis'' integration vector and cloned into a BioBrick with the registry double terminator. | LacI was produced by PCR cloning using Pfu form the ''B. subtilis'' integration vector and cloned into a BioBrick with the registry double terminator. | ||
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Latest revision as of 15:01, 1 October 2008
LacI repressor protein - Terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
LacI was identified from the pDR111 B. subtilis integration vector. The double terminator is the most commonly used registry terminator.
Source
LacI was produced by PCR cloning using Pfu form the B. subtilis integration vector and cloned into a BioBrick with the registry double terminator.