Difference between revisions of "Part:BBa K2927031"
 
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<partinfo>BBa_K2927031 short</partinfo>
 
<partinfo>BBa_K2927031 short</partinfo>
  
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The formation of this six-part DNA sequence for the positive control is derived from six different part sequences of African swine fever virus (ASFV). It is the sequence to test whether our purified Cas12a protein is functioning properly. We used it for positive control in our project.  
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The positive control vector is generated for confirmation of the Cas12a-crRNA activity without using African swine fever virus (ASFV) genome. A fragment containing target sequences of six crRNAs are cloned and amplified by yTA vector.
  
 
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Latest revision as of 06:54, 16 October 2019


Positive control sequence for crRNAs target

The positive control vector is generated for confirmation of the Cas12a-crRNA activity without using African swine fever virus (ASFV) genome. A fragment containing target sequences of six crRNAs are cloned and amplified by yTA vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]