Difference between revisions of "Part:BBa K2927031"

 
 
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<partinfo>BBa_K2927031 short</partinfo>
 
<partinfo>BBa_K2927031 short</partinfo>
  
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We choose the six-part from the African swine fever virus (ASFV) sequence. It is the sequence for testing whether homemade Cas12a protein functions properly. We used it for positive control in our project.  
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The positive control vector is generated for confirmation of the Cas12a-crRNA activity without using African swine fever virus (ASFV) genome. A fragment containing target sequences of six crRNAs are cloned and amplified by yTA vector.
  
 
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Latest revision as of 06:54, 16 October 2019


Positive control sequence for crRNAs target

The positive control vector is generated for confirmation of the Cas12a-crRNA activity without using African swine fever virus (ASFV) genome. A fragment containing target sequences of six crRNAs are cloned and amplified by yTA vector.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]