Difference between revisions of "Part:BBa K3226000"
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− | + | Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E.coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Latest revision as of 06:25, 16 October 2019
This is a device, in which conducts an operation in the cell. This can express the RecA gene.
Deinococcus RecA (DrRecA) protein plays an important role in high radio-resistance of Deinococcus radiodurans. RecA promotes inverse strand exchange reactions and DNA degradation during the early phase of Extended Synthesis Dependent Strand Annealing repair. This gene is homologous E. coli's recA gene. E.coli recA binds preferentially to single strand DNA, on the other hand, Deinococcus RecA binds to double strand DNA, even if single strand DNA is also in the solutions.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2029
Illegal XbaI site found at 2044
Illegal SpeI site found at 3372 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2029
Illegal SpeI site found at 3372
Illegal NotI site found at 2035
Illegal NotI site found at 3379 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2029
Illegal BglII site found at 2499
Illegal XhoI site found at 1013
Illegal XhoI site found at 1905 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2029
Illegal SpeI site found at 3372 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2029
Illegal XbaI site found at 2044
Illegal SpeI site found at 3372
Illegal NgoMIV site found at 3173 - 1000COMPATIBLE WITH RFC[1000]