Difference between revisions of "Part:BBa K3226000:Design"
Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
− | + | Deinococcus radiodurans | |
− | + | ||
===References=== | ===References=== |
Revision as of 06:20, 16 October 2019
This is a device, in which conducts an operation in the cell. This can express the RecA gene.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 2029
Illegal XbaI site found at 2044
Illegal SpeI site found at 3372 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2029
Illegal SpeI site found at 3372
Illegal NotI site found at 2035
Illegal NotI site found at 3379 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2029
Illegal BglII site found at 2499
Illegal XhoI site found at 1013
Illegal XhoI site found at 1905 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2029
Illegal SpeI site found at 3372 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2029
Illegal XbaI site found at 2044
Illegal SpeI site found at 3372
Illegal NgoMIV site found at 3173 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We make this device by using in-fusion cloning. We added homologous sequence between insert and vector by PCR because the primers are needed that sequence in in-fusion cloning.
Source
Deinococcus radiodurans