Difference between revisions of "Part:BBa K2927023"
 
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<partinfo>BBa_K2927023 short</partinfo>
 
<partinfo>BBa_K2927023 short</partinfo>
  
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We changed 5 nucleotides of crRNA 1 from 5’ end to make the mutated crRNA 4. It is the sequence for testing whether homemade Cas12a protein functions properly. We used it for a negative control of our project. Therefore, the sequence of mutated crRNA 4 is too short to become the functional protein. There is no concentration for spread out ASFV of our project.
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To generate negative control vector that cannot bind by crRNA-4, we mutated five nucleotides within the target region of crRNA-4 in positive control vector.  Negative control vector is applied to examine the specify of Cas12a activation.
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 06:01, 16 October 2019

Negative control sequence for crRNA 4 target

To generate negative control vector that cannot bind by crRNA-4, we mutated five nucleotides within the target region of crRNA-4 in positive control vector. Negative control vector is applied to examine the specify of Cas12a activation. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]