Difference between revisions of "Part:BBa K2927022"

 
 
(4 intermediate revisions by one other user not shown)
Line 1: Line 1:
 +
__NOTOC__
 +
<partinfo>BBa_K2927022 short</partinfo>
  
 +
To generate negative control vector that cannot bind by crRNA-3, we mutated five nucleotides within the target region of crRNA-3 in positive control vector.  Negative control vector is applied to examine the specify of Cas12a activation.
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K2927022 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K2927022 parameters</partinfo>
 +
<!-- -->

Latest revision as of 06:00, 16 October 2019

Negative control sequence for crRNA 3 target

To generate negative control vector that cannot bind by crRNA-3, we mutated five nucleotides within the target region of crRNA-3 in positive control vector. Negative control vector is applied to examine the specify of Cas12a activation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]