Difference between revisions of "Part:BBa K2927003"
 
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<partinfo>BBa_K2927003 short</partinfo>
 
<partinfo>BBa_K2927003 short</partinfo>
  
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MBP is the sequence used to translate the maltose-binding protein. Maltose binding protein can be trapped by the MBP column after the TEV enzyme cut the TEV cutting site of the composition (10X His-MBP-TEV-Cas12a). After that, the Cas12a protein will exist in the solution.
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MBP encodes maltose-binding protein. MBP is applied to further purify Cas12a protein by strong binding of Maltose.  
  
 
'''Reference'''<br>
 
'''Reference'''<br>

Latest revision as of 04:43, 16 October 2019


Maltose-binding protein (MBP)

MBP encodes maltose-binding protein. MBP is applied to further purify Cas12a protein by strong binding of Maltose.

Reference
Janice S. Chen, Enbo Ma, Lucas B. Harrington, Maria Da Costa, Xinran Tian, Joel M. Palefsky, Jennifer A. Doudna, Chen et al., Science 360, 436–439 (2018) Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58