Difference between revisions of "Part:BBa K3013000"

(Test edit)
(Test edit)
Line 1: Line 1:
−
 
 
<html>
 
<html>
 +
 +
<!-- This will include a dynamicaly generated element by the page -->
 +
</html><partinfo>BBa_K3013000 short</partinfo><html>
 +
 +
<p>
 +
LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI.
 +
 +
We used this part to tag polypropylene and polystyrene with eGFP.
 +
</p>
 +
 +
 +
===Usage and Biology===
 +
 +
Therefore we made a fusion protein: eGFP - 17x helix - LCI.
 +
This Construct was expressed under a T7 promoter in E. coli BL21. The soluble protein fraction was purified and binding was tested in microtiter plates.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 +
 +
<!-- This will include a dynamicaly generated element by the page -->
 +
</html><partinfo>BBa_K3013000 SequenceAndFeatures</partinfo><html>
 +
 +
 +
===Functional Parameters===
 +
</html><partinfo>BBa_K3013000 parameters</partinfo><html>
 +
 +
<p>
 +
Example Text<br>
 +
<br>
 +
Everything in here works like in a normal website.<br>
 +
So a newline is created with &lt;br&gt;<br>
 +
</p>
 +
<img title="nice title" src="//2019.igem.org/wiki/images/0/0e/T--aachen--favicon.png"/>
 +
<p>
 +
Example Text<br>
 +
<br>
 +
Everything in here works like in a normal website.<br>
 +
So a newline is created with &lt;br&gt;<br>
 +
</p>
  
 
</html>
 
</html>

Revision as of 04:09, 16 October 2019

LCI, polymer binding peptide

LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI. We used this part to tag polypropylene and polystyrene with eGFP.

===Usage and Biology=== Therefore we made a fusion protein: eGFP - 17x helix - LCI. This Construct was expressed under a T7 promoter in E. coli BL21. The soluble protein fraction was purified and binding was tested in microtiter plates. Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]
===Functional Parameters===
biology-NA-
function-NA-
protein-NA-

Example Text

Everything in here works like in a normal website.
So a newline is created with <br>

Example Text

Everything in here works like in a normal website.
So a newline is created with <br>