|
|
| Line 1: |
Line 1: |
| | | | |
| − | __NOTOC__
| + | <html> |
| − | <partinfo>BBa_K3013000 short</partinfo> | + | |
| | | | |
| − | LCI (Liquid Chromatography peak I) is a binding peptide for polystyrene and polypropylene. It can be used to functionalize or tag polymers with proteins fused to the c-terminus of LCI.
| |
| − |
| |
| − | We used this part to tag polypropylene and polystyrene with eGFP.
| |
| − |
| |
| − | <!-- Add more about the biology of this part here
| |
| − | ===Usage and Biology===
| |
| − |
| |
| − | Therefore we made a fusion protein: eGFP - 17x helix - LCI.
| |
| − | This Construct was expressed under a T7 promoter in E. coli BL21. The soluble protein fraction was purified and binding was tested in microtiter plates.
| |
| − |
| |
| − |
| |
| − | <!-- -->
| |
| | <span class='h3bb'>Sequence and Features</span> | | <span class='h3bb'>Sequence and Features</span> |
| − | <partinfo>BBa_K3013000 SequenceAndFeatures</partinfo>
| |
| − |
| |
| | | | |
| − | <!-- Uncomment this to enable Functional Parameter display
| + | </html> |
| − | ===Functional Parameters===
| + | |
| − | <partinfo>BBa_K3013000 parameters</partinfo>
| + | |
| − | <!-- -->
| + | |
