Difference between revisions of "Part:BBa K3195004"
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| − | Similar to subtilisin, our subtilisin-like protease can catalyze the hydrolysis of protein to amino acid. In industry subtilisin has been widely used in all walks of life. Interestingly, we found the subtilisin-like protease can degrade not only proteins but also microcystins. Then we test the activity of this enzyme and measured by HPLC-MS-MS | + | Similar to subtilisin, our subtilisin-like protease can catalyze the hydrolysis of protein to amino acid. In industry subtilisin has been widely used in all walks of life. Interestingly, we found the subtilisin-like protease can degrade not only proteins but also microcystins. Then we test the activity of this enzyme and measured by HPLC-MS-MS. |
| + | [[Image:T--SEU-Nanjing-China--SLPdegraMCRR.png|460px|thumb|center|<b>Figure 1.</b>The abscissa represent 28 enzymes in our project and the unit of ordinate value is mg/L. The ordinate value represents the remained microcystin RR in the degraded sample. The left picture shows the results of degradation by each enzyme, and the right one shows the results of mix each enzyme with enzyme 8(carboxypeptidase Z/N)<b>The sample 12 was degraded by subtilisin-like protease .</b>]] | ||
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Revision as of 00:51, 16 October 2019
Trx-His-EK tagged subtilisin-like protease
Subtilisin-like protease is a kind of alcalase found in amphioxus (Branchiostoma) similar to subtilisin protease. In our project, it has been used to degrade microcystin with another enzyme called carboxypeptidase Z/N.
Usage and Biology
Similar to subtilisin, our subtilisin-like protease can catalyze the hydrolysis of protein to amino acid. In industry subtilisin has been widely used in all walks of life. Interestingly, we found the subtilisin-like protease can degrade not only proteins but also microcystins. Then we test the activity of this enzyme and measured by HPLC-MS-MS.
Figure 1.The abscissa represent 28 enzymes in our project and the unit of ordinate value is mg/L. The ordinate value represents the remained microcystin RR in the degraded sample. The left picture shows the results of degradation by each enzyme, and the right one shows the results of mix each enzyme with enzyme 8(carboxypeptidase Z/N)The sample 12 was degraded by subtilisin-like protease .
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 447
Illegal XhoI site found at 1645 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 889
Illegal AgeI site found at 1120 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 967