Difference between revisions of "Part:BBa K3031018:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
Mutations. See Model.
+
Mutations were made to the amino acid sequence with the aim of improving the reactivity of the reactive cysteine (Cys-199). See our model for the various data we used to determine the parameters used to help improve this part. The main focus was to increase the solvent accessibility of the cysteine.  
  
  

Latest revision as of 00:46, 16 October 2019


OxyR mutated at residues 147 & 203


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 720
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 673


Design Notes

Mutations were made to the amino acid sequence with the aim of improving the reactivity of the reactive cysteine (Cys-199). See our model for the various data we used to determine the parameters used to help improve this part. The main focus was to increase the solvent accessibility of the cysteine.


Source

Biobrock - mutations made with model.

References