Difference between revisions of "Part:BBa K3031018"

 
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<partinfo>BBa_K3031018 short</partinfo>
 
<partinfo>BBa_K3031018 short</partinfo>
  
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OxyR TF
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OxyR Transcription factor protein. OxyR reacts with H2O2 resulting in the oxidation of a reactive cysteine (Cys-199) formation of a intermolecular disulfide bond and thus a conformation change in the OxyR protein. The activated OxyR then binds to certain promoter regions to regulate the expression of downstream genes.
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This is an improvement to the part BBa_K1104200 submitted by National Yang Ming University iGEM Team 2013.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:40, 16 October 2019


OxyR mutated at residues 147 & 203

OxyR Transcription factor protein. OxyR reacts with H2O2 resulting in the oxidation of a reactive cysteine (Cys-199) formation of a intermolecular disulfide bond and thus a conformation change in the OxyR protein. The activated OxyR then binds to certain promoter regions to regulate the expression of downstream genes.

This is an improvement to the part BBa_K1104200 submitted by National Yang Ming University iGEM Team 2013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 720
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 673