Difference between revisions of "Part:BBa K3141000"
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In order to obtain higher proinsulin secretion, we have conducted the optimization of gene expression and insulin secretion using the yeast strain GS115 with pPICZαA plasmid as the expression vector. A interval peptide was introduced between the secretory signal peptides and proinsulin to improve gene expression for the proinsulin secretion.
 
In order to obtain higher proinsulin secretion, we have conducted the optimization of gene expression and insulin secretion using the yeast strain GS115 with pPICZαA plasmid as the expression vector. A interval peptide was introduced between the secretory signal peptides and proinsulin to improve gene expression for the proinsulin secretion.
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<br><p>The amino acid sequence of interval peptide is:EEAEAKR.</p>
+
The amino acid sequence of interval peptide is:EEAEAKR.
  
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<p>Nucleotide sequence is:GAAGAAGCTGAAGCTGAAGCTGAAGCTAAGAGA</p>'''
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Nucleotide sequence is:GAAGAAGCTGAAGCTGAAGCTGAAGCTAAGAGA
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'''
  
  

Revision as of 22:29, 15 October 2019


spacer; increasing proinsulin secretion

Based on others' previous research, we synthesized and modified the spacer sequence to make it more compatible to our project. Spacer BBa_K3141000, a short peptide consisting of 7 amino acids, has been proved that it is able to significantly increase proinsulin expression in Bacillus subtilis and Pichia pastoris through quantitative characterization. When using this spacer, the gene of this spacer is introduced into the N-terminal of HPI gene;then the peptide is synthesized.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]