Difference between revisions of "Part:BBa K3141000"
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| − | + | ===Usage and Biology=== | |
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=== Sequence for interval peptide and proinsulin === | === Sequence for interval peptide and proinsulin === | ||
Revision as of 22:28, 15 October 2019
spacer; increasing proinsulin secretion
Based on others' previous research, we synthesized and modified the spacer sequence to make it more compatible to our project. Spacer BBa_K3141000, a short peptide consisting of 7 amino acids, has been proved that it is able to significantly increase proinsulin expression in Bacillus subtilis and Pichia pastoris through quantitative characterization. When using this spacer, the gene of this spacer is introduced into the N-terminal of HPI gene;then the peptide is synthesized.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]