Difference between revisions of "Part:BBa K2984022:Design"

 
 
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===Design Notes===
 
===Design Notes===
Codonoptimization of part, Use of promoter, terminator respectively that originates from targeted locus.
+
The codon syntax is fitting <i>C. reinhardtii</i>.
  
  
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===Source===
 
===Source===
  
Promoter ist part of 5'UTR of photosystemI reaction center subunit II-1. The terminator originates form 3'UTR Ribulose-1,5-bisphosphat-carboxylase.
 
  
 
===References===
 
===References===
 +
[1]Fischer, N., & Rochaix, J. (2001). The flanking regions of PsaD drive efficient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii. Molecular genetics and genomics, 265, 888-894.

Latest revision as of 20:25, 15 October 2019


PsaD Promoter A1-A3; High-level expression of genes


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 18
    Illegal XhoI site found at 842
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codon syntax is fitting C. reinhardtii.


Source

References

[1]Fischer, N., & Rochaix, J. (2001). The flanking regions of PsaD drive efficient gene expression in the nucleus of the green alga Chlamydomonas reinhardtii. Molecular genetics and genomics, 265, 888-894.